Menopause Transition and Cardiovascular Disease Risk: Implications for Timing of Early Prevention: A Scientific Statement from the American Heart Association. Keywords: PCR-RFLP, Coronary Heart Disease, Gene Polymorphism, VEGF-AĮl Khoudary, S. In conclusion, there was no significant difference in the VEGF-A gene polymorphism in the first generation of CHD and non-CHD groups in Bengkulu City. The statistical analysis results showed that the p-value obtained was 0.460 for the VEGF-A gene polymorphism genotype (rs2010963) in the first CHD and Non-CHD derived samples. The distribution of the VEGF-A genotype (rs2010963) in the first non-CHD offspring, namely GG = 9 (42.9%), CC = 7 (33.3%) and GC = 5 (23.8%). The results of this study indicate that the genotype frequency of the VEGF-A gene polymorphism (rs2010963) in the first generation of patients with CHD is genotype CC = 11 (52.4 %), GG = 7 (33.3%) and CG = 3 (14.3%). This type of research is descriptive observational with a cross-sectional research design. The method used is molecular analysis of polymorphism through Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP). This study aims to identify the VEGF-A gene polymorphism (rs2010963) in the first generation of patients with coronary heart disease (CHD) and non-CHD in Bengkulu City.
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